Taq DNA Polymerase

Taq DNA Polymerase is a thermo stable DNA polymerase that exhibits a 5’→3’ polymerase activity and a 5’→3’exonuclease activity, with no 3’→5’exonuclease activity. Taq DNA Polymerase is purified from an Escherichia coli (E.coli) strain over expressing the gene of Thermus aquaticus DNA polymerase. Taq DNA polymerase is heat-stable and will synthesize DNA at elevated temperatures from single-stranded templates in the presence of a primer. Taq Polymerase is recommended for use in routine PCR reactions. We supplied Taq Polymerase with appropriate buffers. Usually 1-1.5 u of Taq DNA Polymerase is used in 50 µl of reaction mix. Higher Taq DNA Polymerase concentrations may cause synthesis of nonspecific products. However, if inhibitors are present in the reaction mix (e.g., if the template DNA used is not highly purified), higher amounts of Taq DNA polymerase (2-3 u) may be necessary to obtain a better yield of amplification products. No endonuclease, exonuclease, or bacterial DNA were detected in this kit.