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2 x SYBR Green qPCR Mix provides rapid real-time quantification of RNA targets in SYBR Green fluorescent. The components of 2 x SYBR Green qPCR Mix include Hot-start enzyme named HotMaster Taq DNA Polymerase, dNTP, PCR reaction buffer, BSA and SYBR Green , and so on. The fluorescent dye SYBR Green I in the master mix enables the analysis of many different targets without having to synthesize target specific labeled probes.
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SYBR Green Master Mix Components
SYBR Green master mixes are 2x concentrated, and contain dNTPs, MgCl2, and DNA polymerase. SYBR Green master mixes are available with other dyes including ROX. Many PCR machines require a passive reference dye. An internal reference dye, such as ROX, corrects well-to-well optical variations, and is used for fluorescent signal normalization.