RNA Isolater Total RNA Extraction Reagent

Features

RNA Isolater Total RNA Extraction Reagent uses guanidinium isothiocyanate and phenol, with high lysis ability. It can lyse cells and tissues in short time, keeping integrity of RNA. The RNA isolater Total RNA Extraction Reagent is widely used for RNA extraction of cultured cells, animal tissues, microorganisms, and plant tissues with weak secondary metabolism (i.e. seedling and spire). Samples will be lysed thoroughly in RNA isolater and form supernatant, interlayer, and organic layer (bright red sublayer). RNA is distributed in the upper water phase. Remove the RNA-containing supernatant layer and treat with isopropanol to extract the total RNA.

Tissue & Cultured cells

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Homogenize in RNA isolater or triturate in

liquid nitrogen and dissolve by RNA isolater.

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Add Chloroform of 1/5 solution volume, mix vigorously,

and place on ice (4℃) for 5 min.

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Centrifuge at 12,000 × g for 15 min at 4℃.

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Transfer the supernatant to a new tube, add equal volume of

isopropanol, and place on ice (4℃) for 10 min.

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Centrifuge at 12,000 × g for 10 min at 4℃.

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Rinse with 75% ethanol.

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Centrifuge at 12,000 × g for 5 min at 4℃.

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Discard the supernatant, dry the RNA-containing pellet,

and dissolve the pellet with DEPC treated water.

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Test the integrity of RNA using gel electrophoresis,

test the purity and concentration with a spectrophotometer.