Nile Blue Chloride

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Dye Content (by Spectrophotometry)>90%
Absorption maximum (50% EtOH) ?max635-639nm
M.F.C20H20ClN3O
Absorptivity (A1%/1cm in 50% EtOH, at ?max)>1800
Supply TypeManufacturer, Supplier
Preferred Buyer Location All over the world

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Company Information

GSP Chemical Industries

Palghar, Maharashtra

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Member Since 12 Years
Nature of Business Retailer
Year of Establishment 1981

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Solubility 0.1% (50% EtOH)Clear solution
CI NO.51180
Absorptivity (A1%/1cm in 50% EtOH, at ?max)>1800
M.F.C20H20ClN3O
Loss On Drying (110°C)<8%
Absorption maximum (50% EtOH) ?max635-639nm
M.W.353.85gm/mole
Dye Content (by Spectrophotometry)>90%
C.A.S. No.2381-85-3

Chemical name

Bis(5-amino-9-(diethylamino)benzo(a)phenoxazin-7-ium) chloride

InChI Key

SHXOKQKTZJXHHR-UHFFFAOYSA-N

Solubility

Soluble in ethyl alcohol

Nile blue chloride has been used to stain non-aqueous phase in fluorescence microscopy studies. Biochem/physiol Actions. Nile blue chloride is a green colored powder and is a member of oxazine group of dyes. It changes its color from blue (pH 9.4) to purple-red (11.0).

Applications:

Nile Blue chloride, or Nile Blue A, is a fluorescence reference standard used for various histologic staining. It is commonly utilized in studies involved with lipid and nuclei staining. Nile Blue chloride imparts a pink color to unsaturated glycerides and neutral lipids and stains nuclei and acids (such as fatty acids, chormolipids and phospholipids) a purple-blue color.

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