Mutagenesis Kit

Other Details :

• Thermo Scientific Phusion Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing point mutations, insertions, or deletions in any type of plasmid DNA
• With this kit, the entire plasmid is amplified using phosphorylated primers that introduce the desired changes
• The amplified, linear PCR product, containing the desired mutation, is circularized in a 5-minute ligation reaction with T4 DNA Ligase
• The resulting plasmid can be then transformed into any competent
• The optimal annealing temperature for Phusion DNA Polymerases may differ significantly from that of Taq-based polymerases

Features :

• Excellent quality
• Air tight packaging
• Purity

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