JSB I & II Malaria Stain Kit

Composition information

• Stains supplied: Cat. no. HE 807
• Preparation of working reagent: All reagents are ready to use

Rapid-malaria JSBI 500 ml

• Methylene blue: 4.3 m Mol/L
• Sulphuric acid: 1%
• Potassium dichromate: 0.5 gms
• Phosphate buffer: 0.1 m Mol
• Contains polychromating materials, preservatives and stabilizers

Rapid-malaria JSB II 500 ml

• Eosin: 4.3 m Mol/L
• Phosphate: 0.1 m Mol/L
• Contains preservatives and stabilizers
• Easyfix 50 ml
• Peripheral smear fixative

Stability

• All reagents are stable between 25 to 30 degree C for 60 months
• Rapid-malaria fixative must be stored tight capped away from heat and fire
• Rapid-malaria JSB stain are exhausted on prolonged use over 150 smears
• Advised to discard rapid-malaria JSB Stain as and when the smear gets lighter stain

Samples

• Peripheral blood samples on clean glass slides may be collected upon febrile episodes from susceptible patients
• Avoid anticoagulated samples

Thin Smear

• A drop of blood can be spread with a fine edge spreader
• Dry the smear at air and fix it with fixative or rectified spirit within 4 hours
• Fixed smear can be stored for longer period without much variation in staining result
• Preferable to stain all smears as early as possible

Thick Smears

• In mass or conclusive screening it is preferable to prepare thick smears by collecting 1-2 drop of blood on a clean glass slide and spread it to the shape of a coin
• Dry at air, add few drops of distilled water to the slide to cover the smears and wait for 1-2 minutes to dehemoglobinized the smear
• Carefully remove water and dry at air
• Fix the smear