Preparative HPLC is used for the isolation and purification of valuable products in the chemicals and pharmaceutical industries as well as biotechnology and biochemistry .Processes of preparative chromatography depend upon, the nature of compound and .amount of compound to be isolated. It starts in the μg range for isolation of enzymes in biotechnology and few milligrams in natural products. Industrial scale or production scale preparative HPLC are basic tool to offer highly purified products at economical price.