Analytical techniques have been popularly used in quality testing for pharmaceuticals and food products. With their advent in diagnostics, the avenue and scope of testing widened and ability to screen and quantify analytes in nanogram became a possibility. When molecules in the mixture are very similar, direct quantification becomes difficult. HPLC is a form of column chromatography used frequently to separate, identify and quantify compounds. It consists of a stationary phase that absorbs the analytes and holds them for a particular time.

Analytical techniques have been popularly used in quality testing for pharmaceuticals and food products. With their advent in diagnostics, the avenue and scope of testing widened and ability to screen and quantify analytes in nanogram became a possibility. When molecules in the mixture are very similar, direct quantification becomes difficult. HPLC is a form of column chromatography used frequently to separate, identify and quantify compounds. It consists of a stationary phase that absorbs the analytes and holds them for a particular time.