Haematoxylin - also called natural black 1 or C.I. 75290, is a compound Extracted from the bark of the logwood tree. Hematoxylin is a basic and positively charged compound that binds to and forms salts with acidic (basophilic) compounds containing negative charges (such as DNA and RNA, which are acidic/negative because the nucleic acid building blocks that come off the phosphate backbone are negatively charged) and stains them dark blue or violet.