DNA Loading Dye

Features

6X BX/Loading Buffer is used as a loading dye for visual tracking of DNA migration during electrophoresis. It incorporates Bromophenol blue and Xylene Cyanol FF as tracking dye. Bromophenol blue migrates fast in the agarose gel and corresponds to the migration of a 300 - 500 bp long DNA fragment in a 1% agarose gel. Xylene cyanol FF migrates comparatively slower and corresponds to the migration of a 4,000 –5,000 bp long DNA fragment in a 1% agarose gel.

Advantages & Features :

• Two-colour tracking of DNA migration during DNA electrophoresis
• No DNA masking during gel exposure to UV light
• EDTA binds divalent metal ions and inhibits metal dependent nucleases 

Applications :

Preparation of DNA ladders, markers, and samples for loading on agarose or polyacrylamide gels