Cell Culture Media

Good cell culture and laboratory practices,Cell culture media formulations and preparations,Cell culture techniques: Revival of frozen cells, subculture of monolayer and suspension cells, cell counting and cryopreservation,Cell viability and proliferation assays,Cell culture contaminants identification and control,Isolation and culture of cells from primary tumours and human vessels (based on availability),Detection of cell death mechanism (apoptosisnecrosis),Cell cycle analysis,Immunostaining techniques using fluorescent microscopy and flow cytometry