Specifications

• Gram negative bacteria stain is red that is attributed to thinner peptidoglycan wall that does not retain crystal violet during DE-coloring process
• Primarily, the test detects peptidoglycan that is present in thick layer in Gram positive bacteria
• Gram stain is always first step in identification of bacterial organism.
• Used as a technique for differentiating two large groups of bacteria based on their different cell wall constituents
• Procedure distinguishes between Gram positive and Gram negative groups through coloring these cells in red or violet
• Gram positive bacteria stain violet because of thick layer of peptidoglycan in their cell walls that retains crystal violet

Specifications

• Gram negative bacteria stain is red that is attributed to thinner peptidoglycan wall that does not retain crystal violet during DE-coloring process
• Primarily, the test detects peptidoglycan that is present in thick layer in Gram positive bacteria
• Gram stain is always first step in identification of bacterial organism.
• Used as a technique for differentiating two large groups of bacteria based on their different cell wall constituents
• Procedure distinguishes between Gram positive and Gram negative groups through coloring these cells in red or violet
• Gram positive bacteria stain violet because of thick layer of peptidoglycan in their cell walls that retains crystal violet