Features

RNase A (ribonuclease A) is a bovine pancreatic endoribonuclease that cleaves single-stranded RNA. It catalyzes the cleavage of the phosphodiester bond between the 5’-ribose of a nucleotide and the phosphate group attached to the 3’-ribose of an adjacent pyrimidine nucleotide. This cleavage forms a 2’, 3’-cyclic phosphate, which is then hydrolysed to the corresponding 3’-nucleoside phosphate.

Advantages & Features :

• Small single-chain polypeptide : 124 residues, ~13.7 kDa.
• Avoids the use of co-factors and divalent cations.
• High specific activity : > 100 Kunitz units per mg protein.
• High Quality : free of DNase, Proteinase or exonuclease contamination.
• Time-saving protocol : avoids to RNAse heat step before use. 

Applications :

• RNA removal during DNA isolation.
• RNA sequence analysis.
• RNase protection assays.
• RNA quantification or mapping.
• Purifying plasmid DNA.
• Genomic DNA isolation.
• Molecular weight marker.

Features

RNase A (ribonuclease A) is a bovine pancreatic endoribonuclease that cleaves single-stranded RNA. It catalyzes the cleavage of the phosphodiester bond between the 5’-ribose of a nucleotide and the phosphate group attached to the 3’-ribose of an adjacent pyrimidine nucleotide. This cleavage forms a 2’, 3’-cyclic phosphate, which is then hydrolysed to the corresponding 3’-nucleoside phosphate.

Advantages & Features :

• Small single-chain polypeptide : 124 residues, ~13.7 kDa.
• Avoids the use of co-factors and divalent cations.
• High specific activity : > 100 Kunitz units per mg protein.
• High Quality : free of DNase, Proteinase or exonuclease contamination.
• Time-saving protocol : avoids to RNAse heat step before use. 

Applications :

• RNA removal during DNA isolation.
• RNA sequence analysis.
• RNase protection assays.
• RNA quantification or mapping.
• Purifying plasmid DNA.
• Genomic DNA isolation.
• Molecular weight marker.